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| カタログ番号 | HP166014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human METTL1 (Thr34-His276). | ||||||||
| ターゲット | miRNA (guanine-N(7)-)-methyltransferase, Methyltransferase-like protein 1, tRNA (guanine-N(7)-)-methyltransferase, mRNA (guanine-N(7)-)-methyltransferase, C12orf1, tRNA (guanine(46)-N(7))-methyltransferase, METTL1, tRNA(m7G46)-methyltransferase | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q9UBP6 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | tRNA (guanine-N(7)-)-methyltransferase (METTL1) is a ~31 kDa protein. Catalytic component of METTL1-WDR4 methyltransferase complex that mediates the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs). Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in a large subset of tRNAs that contain the 5'-RAGGU-3' motif within the variable loop. M7G46 interacts with C13-G22 in the D-loop to stabilize tRNA tertiary structure and protect tRNAs from decay. Also acts as a methyltransferase for a subset of internal N(7)-methylguanine in mRNAs. Internal N(7)-methylguanine methylation of mRNAs in response to stress promotes their relocalization to stress granules, thereby suppressing their translation. 1. Alexandrov, A. et al. (2002) RNA (New York, N.Y.) 8, 1253-66. PMID: 12403464 2. Pandolfini, L. et al. (2019) Molecular cell 74, 1278-1290.e9. PMID: 31031083 3. Zhang, LS. et al. (2019) Molecular cell 74, 1304-1316.e8. PMID: 31031084 4. Ruiz-Arroyo, VM. et al. (2023) Nature 613, 383-390. PMID: 36599982 5. Li, J. et al. (2023) Nature 613, 391-397. PMID: 36599985 6. Jin, X. et al. (2023) Cell discovery 9, 65. PMID: 37369656 7. Zhao, Z. et al. (2023) Cell 186, 3208-3226.e27. PMID: 37379838 8. Dai, Z. et al. (2021) Molecular cell 81, 3339-3355.e8. PMID: 34352206 9. Orellana, EA. et al. (2021) Molecular cell 81, 3323-3338.e14. PMID: 34352207 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-METTL1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: A549 cell lysate
Lane 2: 293T cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Jurkat cell lysate
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