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| カタログ番号 | HV489014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human METTL3 Polyclonal Antibody (HV489014) is a rabbit polyclonal antibody detecting METTL3 in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human METTL3 (Met1-Leu580). | ||||||||
| ターゲット | METTL3, N6-adenosine-methyltransferase 70 kDa subunit, N6-adenosine-methyltransferase catalytic subunit, MTA70, Methyltransferase-like protein 3, MT-A70, hMETTL3 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q86U44 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | N(6)-adenosine-methyltransferase catalytic subunit METTL3 is a ~64 kDa protein. The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some RNAs and regulates various processes such as the circadian clock, differentiation of embryonic and hematopoietic stem cells, cortical neurogenesis, response to DNA damage, differentiation of T-cells and primary miRNA processing. In the heterodimer formed with METTL14, METTL3 constitutes the catalytic core. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability, processing, translation efficiency and editing. M6A acts as a key regulator of mRNA stability: methylation is completed upon the release of mRNA into the nucleoplasm and promotes mRNA destabilization and degradation. In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization, promoting differentiation of ESCs. 1. Dominissini, D. et al. (2012) Nature 485, 201-6. PMID: 22575960 2. Wang, X. et al. (2014) Nature 505, 117-20. PMID: 24284625 3. Liu, N. et al. (2015) Nature 518, 560-4. PMID: 25719671 4. Alarcón, CR. et al. (2015) Nature 519, 482-5. PMID: 25799998 5. Alarcón, CR. et al. (2015) Cell 162, 1299-308. PMID: 26321680 6. Meyer, KD. et al. (2015) Cell 163, 999-1010. PMID: 26593424 7. Wang, X. et al. (2016) Nature 534, 575-8. PMID: 27281194 8. Wang, P. et al. (2016) Molecular cell 63, 306-317. PMID: 27373337 9. Śledź, P. et al. (2016) eLife 5. PMID: 27627798 10. Xiang, Y. et al. (2017) Nature 543, 573-576. PMID: 28297716 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-METTL3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K562 cell lysate
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