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Anti-Human METTL5 Polyclonal Antibody (HA273014)

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Anti-Human METTL5 Polyclonal Antibody
Anti-Human METTL5 Polyclonal Antibody
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Vista general
Número de catálogoHA273014
Descripción
Anti-Human METTL5 Polyclonal Antibody (HA273014) is a rabbit polyclonal antibody detecting METTL5 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human METTL5 (Met1-Phe209).
Objetivo Methyltransferase-like protein 5, METTL5, rRNA N6-adenosine-methyltransferase METTL5
Purificación Purified by antigen affinity column.
Número de acceso Q9NRN9
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

rRNA N(6)-adenosine-methyltransferase METTL5 is a ~23 kDa protein. Catalytic subunit of a heterodimer with TRMT112, which specifically methylates the 6th position of adenine in position 1832 of 18S rRNA. N6-methylation of adenine(1832) in 18S rRNA resides in the decoding center of 18S rRNA and is required for translation and embryonic stem cells (ESCs) pluripotency and differentiation.

1. van Tran, N. et al. (2019) Nucleic acids research 47, 7719-7733. PMID: 31328227
2. Ignatova, VV. et al. (2020) Genes & development 34, 715-729. PMID: 32217665
3. Rong, B. et al. (2020) Cell reports 33, 108544. PMID: 33357433
4. Yu, D. et al. (2021) The Journal of biological chemistry 296, 100270. PMID: 33428944
5. Sepich-Poore, C. et al. (2022) The Journal of biological chemistry 298, 101590. PMID: 35033535
Nota For research use only.
Imágenes
  • Anti-Human METTL5 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-METTL5 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: K562 cell lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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