Anti-Human METTL5 Polyclonal Antibody (HA273014)

Name: Anti-Human METTL5 Polyclonal Antibody
Brand: abinScience
SKU: HA273014
Price: 96 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HA273014

説明

Anti-Human METTL5 Polyclonal Antibody (HA273014) is a rabbit polyclonal antibody detecting METTL5 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

種反応性

Human, Mouse

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human METTL5 (Met1-Phe209).

ターゲット

Methyltransferase-like protein 5, METTL5, rRNA N6-adenosine-methyltransferase METTL5

精製

Purified by antigen affinity column.

アクセッション番号

Q9NRN9

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

rRNA N(6)-adenosine-methyltransferase METTL5 is a ~23 kDa protein. Catalytic subunit of a heterodimer with TRMT112, which specifically methylates the 6th position of adenine in position 1832 of 18S rRNA. N6-methylation of adenine(1832) in 18S rRNA resides in the decoding center of 18S rRNA and is required for translation and embryonic stem cells (ESCs) pluripotency and differentiation.

1. van Tran, N. et al. (2019) Nucleic acids research 47, 7719-7733. PMID: 31328227

2. Ignatova, VV. et al. (2020) Genes & development 34, 715-729. PMID: 32217665

3. Rong, B. et al. (2020) Cell reports 33, 108544. PMID: 33357433

4. Yu, D. et al. (2021) The Journal of biological chemistry 296, 100270. PMID: 33428944

5. Sepich-Poore, C. et al. (2022) The Journal of biological chemistry 298, 101590. PMID: 35033535

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-METTL5 polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate

参考文献

データシート

Anti-Human METTL5 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-Human METTL5 Polyclonal Antibody (HA273014)

概要

イメージ

参考文献

データシート

SDS