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Anti-Human METTL5 Polyclonal Antibody
Anti-Human METTL5 Polyclonal Antibody
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Спецификация:
  • 20ug
  • 50ug
  • 100ug
Количество:
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Обзор
Каталожный номерHA273014
Описание
Anti-Human METTL5 Polyclonal Antibody (HA273014) is a rabbit polyclonal antibody detecting METTL5 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовHuman, Mouse
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human METTL5 (Met1-Phe209).
Цель Methyltransferase-like protein 5, METTL5, rRNA N6-adenosine-methyltransferase METTL5
Очистка Purified by antigen affinity column.
Номер доступа Q9NRN9
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

rRNA N(6)-adenosine-methyltransferase METTL5 is a ~23 kDa protein. Catalytic subunit of a heterodimer with TRMT112, which specifically methylates the 6th position of adenine in position 1832 of 18S rRNA. N6-methylation of adenine(1832) in 18S rRNA resides in the decoding center of 18S rRNA and is required for translation and embryonic stem cells (ESCs) pluripotency and differentiation.

1. van Tran, N. et al. (2019) Nucleic acids research 47, 7719-7733. PMID: 31328227
2. Ignatova, VV. et al. (2020) Genes & development 34, 715-729. PMID: 32217665
3. Rong, B. et al. (2020) Cell reports 33, 108544. PMID: 33357433
4. Yu, D. et al. (2021) The Journal of biological chemistry 296, 100270. PMID: 33428944
5. Sepich-Poore, C. et al. (2022) The Journal of biological chemistry 298, 101590. PMID: 35033535
Примечание For research use only.
Изображения
  • Anti-Human METTL5 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-METTL5 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: K562 cell lysate

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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