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Каталожный номерHP691014
Описание
Anti-Human MICAL1 Polyclonal Antibody (HP691014) is a rabbit polyclonal antibody detecting MICAL1 in ELISA, IHC, WB. Suitable for Mouse, Bovine, Human, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human MICAL1 (Met1-Lys488).
Цель NICAL, [F-actin]-monooxygenase MICAL1, MICAL1, MICAL-1, NEDD9-interacting protein with calponin homology and LIM domains, Molecule interacting with CasL protein 1, MICAL
Очистка Purified by antigen affinity column.
Номер доступа Q8TDZ2
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

[F-actin]-monooxygenase MICAL1 is a ~117 kDa protein. Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization. In the absence of actin, it also functions as a NADPH oxidase producing H(2)O(2). Acts as a cytoskeletal regulator that connects NEDD9 to intermediate filaments. Also acts as a negative regulator of apoptosis via its interaction with STK38 and STK38L; acts by antagonizing STK38 and STK38L activation by MST1/STK4. Involved in regulation of lamina-specific connectivity in the nervous system such as the development of lamina-restricted hippocampal connections.

1. Wu, H. et al. (2018) Scientific reports 8, 937. PMID: 29343822
2. Zucchini, D. et al. (2011) Archives of biochemistry and biophysics 515, 1-13. PMID: 21864500
3. Vitali, T. et al. (2016) Archives of biochemistry and biophysics 593, 24-37. PMID: 26845023
4. Lucken-Ardjomande Häsler, S. et al. (2020) The Journal of cell biology 219. PMID: 32344433
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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