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Anti-Human MIF Recombinant Antibody (SAA2333) (HB816013)

Anti-Human MIF Recombinant Antibody (SAA2333)
Anti-Human MIF Recombinant Antibody (SAA2333)
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Aperçu
Numéro de catalogueHB816013
Description
Anti-Human MIF Recombinant Antibody (SAA2333) (HB816013) is a mouse monoclonal antibody detecting MIF in ELISA, Blocking. Suitable for Human, Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, Blocking
Espèce hôteMouse
ClonalitéMonoclonal
IsotypeIgG2a, kappa
ID de cloneSAA2333
Cible Macrophage migration inhibitory factor, MIF, EC:5.3.2.1, Glycosylation-inhibiting factor, GIF, L-dopachrome isomerase, L-dopachrome tautomerase, EC:5.3.3.12, Phenylpyruvate tautomerase, MIF, GLIF, MMIF
Niveau d'endotoxines < 10 EU/mg as determined by LAL method.
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès P14174, P34884
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:2000-1:20000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Macrophage migration inhibitory factor (MIF) is a ~12 kDa protein. Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.

1. Oddo, M. et al. (2005) Infection and immunity 73, 3783-6. PMID: 15908412
2. Emonts, M. et al. (2007) Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 44, 1321-8. PMID: 17443469
3. Fan, C. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 10994-9. PMID: 23776208
4. Tan, TH. et al. (2001) The Biochemical journal 357, 373-83. PMID: 11439086
Note For research use only.
Isotype Control Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)
Images
  • Anti-Human MIF Recombinant Antibody (SAA2333)

    SDS-PAGE

    SDS-PAGE for Anti-Human MIF Antibody (SAA2333)

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
Recommandation

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