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Anti-Human MPHOSPH10 Polyclonal Antibody (HT215014)

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Anti-Human MPHOSPH10 Polyclonal Antibody
Anti-Human MPHOSPH10 Polyclonal Antibody
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Vista general
Número de catálogoHT215014
Descripción
Anti-Human MPHOSPH10 Polyclonal Antibody (HT215014) is a rabbit polyclonal antibody detecting MPHOSPH10 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human MPHOSPH10 (Asp461-Leu681).
Objetivo U3 small nucleolar ribonucleoprotein protein MPP10, M phase phosphoprotein 10, MPHOSPH10, MPP10
Purificación Purified by antigen affinity column.
Número de acceso O00566
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

U3 small nucleolar ribonucleoprotein protein MPP10 (MPHOSPH10) is a ~78 kDa protein. Component of the 60-80S U3 small nucleolar ribonucleoprotein (U3 snoRNP). Required for the early cleavages during pre-18S ribosomal RNA processing. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.

1. Granneman, S. et al. (2003) Nucleic acids research 31, 1877-87. PMID: 12655004
2. Singh, S. et al. (2021) Science (New York, N.Y.) 373, eabj5338. PMID: 34516797
Nota For research use only.
Imágenes
  • Anti-Human MPHOSPH10 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-MPHOSPH10 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Jurkat cell lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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