Anti-Human MT-ATP8 Polyclonal Antibody (HY325014)

Name: Anti-Human MT-ATP8 Polyclonal Antibody
Brand: abinScience
SKU: HY325014
Price: 96 USD
Availability: InStock

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Fiche technique
SDS

Aperçu

Numéro de catalogue

HY325014

Description

Anti-Human MT-ATP8 Polyclonal Antibody (HY325014) is a rabbit polyclonal antibody detecting MT-ATP8 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human MT-ATP8 (Gln25-Ser68).

Cible

ATP synthase protein 8, A6L, F-ATPase subunit 8, MT-ATP8, ATP8, ATPASE8, MTATP8

Purification

Purified by antigen affinity column.

Numéro d'accès

P03928

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

ATP synthase F(0) complex subunit 8 (MT-ATP8) is a ~7 kDa protein. Subunit 8, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro.

1. Lai, Y. et al. (2023) Molecular cell 83, 2137-2147.e4. PMID: 37244256

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MT-ATP8 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: Lncap cell lysate
Lane 3: A549 cell lysate
Lane 4: Hela cell lysate

Reférences

Fiche technique

Anti-Human MT-ATP8 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-Human MT-ATP8 Polyclonal Antibody (HY325014)

Aperçu

Images

Reférences

Fiche technique

SDS