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| カタログ番号 | HY325014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human MT-ATP8 Polyclonal Antibody (HY325014) is a rabbit polyclonal antibody detecting MT-ATP8 in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human MT-ATP8 (Gln25-Ser68). | ||||||||
| ターゲット | ATP synthase protein 8, A6L, F-ATPase subunit 8, MT-ATP8, ATP8, ATPASE8, MTATP8 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P03928 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | ATP synthase F(0) complex subunit 8 (MT-ATP8) is a ~7 kDa protein. Subunit 8, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. 1. Lai, Y. et al. (2023) Molecular cell 83, 2137-2147.e4. PMID: 37244256 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-MT-ATP8 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: Lncap cell lysate
Lane 3: A549 cell lysate
Lane 4: Hela cell lysate
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