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Anti-Human MTAP Polyclonal Antibody (HC278014)

Anti-Human MTAP Polyclonal Antibody
Anti-Human MTAP Polyclonal Antibody
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Vista general
Número de catálogoHC278014
Descripción
Anti-Human MTAP Polyclonal Antibody (HC278014) is a rabbit polyclonal antibody detecting MTAP in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Macaca mulatta.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human MTAP (Met1-His283).
Objetivo MTAP, S-methyl-5'-thioadenosine phosphorylase, MSAP, MTA phosphorylase, 5'-methylthioadenosine phosphorylase, MTAPase
Purificación Purified by antigen affinity column.
Número de acceso Q13126
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

S-methyl-5'-thioadenosine phosphorylase (MTAP) is a ~31 kDa protein. Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.

Nota For research use only.
Imágenes
  • Anti-Human MTAP Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-MTAP polyclonal antibody at 1μg/ml on various samples.
    Lane 1: NIH3T3 cell lysate
    Lane 2: HT-29 cell lysate
    Lane 3: Raji cell lysate
    Lane 4: Hela cell lysate
    Lane 5: HCT116 cell lysate

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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