Anti-Human MTHFD1 Polyclonal Antibody (HY454014)

Name: Anti-Human MTHFD1 Polyclonal Antibody
Brand: abinScience
SKU: HY454014
Price: 96 USD
Availability: InStock

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HY454014

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human MTHFD1 (Met1-Lys297).

Cible

MTHFC, C1-THF synthase, MTHFD1, C-1-tetrahydrofolate synthase, cytoplasmic, MTHFD

Purification

Purified by antigen affinity column.

Numéro d'accès

P11586

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

C-1-tetrahydrofolate synthase, cytoplasmic (MTHFD1) is a ~101 kDa protein. Trifunctional enzyme that catalyzes the interconversion of three forms of one-carbon-substituted tetrahydrofolate: (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate, 5,10-methenyltetrahydrofolate and (6S)-10-formyltetrahydrofolate. These derivatives of tetrahydrofolate are differentially required in nucleotide and amino acid biosynthesis, (6S)-10-formyltetrahydrofolate being required for purine biosynthesis while (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate is used for serine and methionine biosynthesis for instance.

1. Schmidt, A. et al. (2000) Biochemistry 39, 6325-35. PMID: 10828945

2. Christensen, KE. et al. (2009) Human mutation 30, 212-20. PMID: 18767138

3. Hum, DW. et al. (1991) Protein engineering 4, 493-500. PMID: 1881876

4. Burda, P. et al. (2015) Journal of inherited metabolic disease 38, 863-72. PMID: 25633902

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MTHFD1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: 293T cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K562 cell lysate
Lane 4: Hela cell lysate
Lane 5: A549 cell lysate
Lane 6: Mouse kidney lysate
Lane 7: Mouse liver lysate
Lane 8: Rat liver lysate

Reférences

Fiche technique

Anti-Human MTHFD1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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