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| カタログ番号 | HY454014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human MTHFD1 (Met1-Lys297). | ||||||||
| ターゲット | MTHFC, C1-THF synthase, MTHFD1, C-1-tetrahydrofolate synthase, cytoplasmic, MTHFD | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P11586 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | C-1-tetrahydrofolate synthase, cytoplasmic (MTHFD1) is a ~101 kDa protein. Trifunctional enzyme that catalyzes the interconversion of three forms of one-carbon-substituted tetrahydrofolate: (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate, 5,10-methenyltetrahydrofolate and (6S)-10-formyltetrahydrofolate. These derivatives of tetrahydrofolate are differentially required in nucleotide and amino acid biosynthesis, (6S)-10-formyltetrahydrofolate being required for purine biosynthesis while (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate is used for serine and methionine biosynthesis for instance. 1. Schmidt, A. et al. (2000) Biochemistry 39, 6325-35. PMID: 10828945 2. Christensen, KE. et al. (2009) Human mutation 30, 212-20. PMID: 18767138 3. Hum, DW. et al. (1991) Protein engineering 4, 493-500. PMID: 1881876 4. Burda, P. et al. (2015) Journal of inherited metabolic disease 38, 863-72. PMID: 25633902 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-MTHFD1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: 293T cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K562 cell lysate
Lane 4: Hela cell lysate
Lane 5: A549 cell lysate
Lane 6: Mouse kidney lysate
Lane 7: Mouse liver lysate
Lane 8: Rat liver lysate
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