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| カタログ番号 | HB832014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human MTHFD2 Polyclonal Antibody (HB832014) is a rabbit polyclonal antibody detecting MTHFD2 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Chicken.
Highlights
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| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human MTHFD2 (Glu36-Asn350). | ||||||||
| ターゲット | NMDMC, MTHFD2, Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P13995 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial (MTHFD2) is a ~37 kDa protein. Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency. | ||||||||
| 注意事項 | For research use only. |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting MTHFD2 (HB832014) at a concentration of 10.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of MTHFD2 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
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