Anti-Human MTHFD2 Polyclonal Antibody (HB832014)

Name: Anti-Human MTHFD2 Polyclonal Antibody
Brand: abinScience
SKU: HB832014
Price: 96 USD
Availability: InStock

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Каталожный номер

HB832014

Описание

Anti-Human MTHFD2 Polyclonal Antibody (HB832014) is a rabbit polyclonal antibody detecting MTHFD2 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human MTHFD2 (Glu36-Asn350).

Цель

NMDMC, MTHFD2, Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial

Очистка

Purified by antigen affinity column.

Номер доступа

P13995

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial (MTHFD2) is a ~37 kDa protein. Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency.

Примечание

For research use only.

Изображения

Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting MTHFD2 (HB832014) at a concentration of 10.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of MTHFD2 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

Ссылки

Даташит

Anti-Human MTHFD2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

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Anti-Human MTHFD2 Polyclonal Antibody (HB832014)

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Изображения

Ссылки

Даташит

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