

| Número de catálogo | HB102137 |
|---|---|
| Reactividad de especies | Human |
| Aplicaciones | ELISA, FCM, WB |
| Especie huésped | Human |
| Clonalidad | Monoclonal |
| Isotipo | IgG1 |
| ID de clon | 5E5# |
| Conjugación | APC |
| Aplicaciones probadas | FCM |
| Objetivo | Breast carcinoma-associated antigen DF3, CA 15-3, CD227, Cancer antigen 15-3, Carcinoma-associated mucin, EMA, Episialin, H23AG, KL-6, Krebs von den Lungen-6, MUC-1, MUC1, MUC1-CT, MUC1-NT, MUC1-alpha, MUC1-beta, Mucin-1, Mucin-1 subunit alpha, Mucin-1 subunit beta, PEM, PEMT, PUM, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin |
| Nivel de endotoxinas | Please contact with the lab for this information. |
| Pureza | >90% as determined by SDS-PAGE. |
| Productos asociados | Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), APC |
| Número de acceso | P15941 |
| Forma | Liquid |
| Buffer de almacenamiento | 0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote. |
| Estabilidad y almacenamiento | Store at 4°C for 12 months. Protect from light. Do not freeze. |
| Fondo | Mucin-1 (CD227/MUC1) is a ~122 kDa protein. The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. |
| Nota | For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only. |

Flow-cytometry using APC anti-human CD227 antibody. MCF-7 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD227 monoclonal antibody (Catalog: HB102137, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.




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