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Anti-Human MUC-1 (Tn/STn) Recombinant Antibody (5E5#), APC (HB102137)

Anti-Human MUC-1 (Tn/STn) Recombinant Antibody (5E5#), APC
Anti-Human MUC-1 (Tn/STn) Recombinant Antibody (5E5#), APC
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Aperçu
Numéro de catalogueHB102137
Reactivité des espècesHuman
ApplicationsELISA, FCM, WB
Espèce hôteHuman
ClonalitéMonoclonal
IsotypeIgG1
ID de clone5E5#
ConjugaisonAPC
Applications testées FCM
Cible Breast carcinoma-associated antigen DF3, CA 15-3, CD227, Cancer antigen 15-3, Carcinoma-associated mucin, EMA, Episialin, H23AG, KL-6, Krebs von den Lungen-6, MUC-1, MUC1, MUC1-CT, MUC1-NT, MUC1-alpha, MUC1-beta, Mucin-1, Mucin-1 subunit alpha, Mucin-1 subunit beta, PEM, PEMT, PUM, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Produits associés Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), APC
Numéro d'accès P15941
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Store at 4°C for 12 months. Protect from light. Do not freeze.
Contexte

Mucin-1 (CD227/MUC1) is a ~122 kDa protein. The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.

Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Images
  • Anti-Human MUC-1 (Tn/STn) Recombinant Antibody (5E5#), APC

    Flow CytoMetry

    Flow-cytometry using APC anti-human CD227 antibody. MCF-7 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD227 monoclonal antibody (Catalog: HB102137, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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