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| カタログ番号 | HF552014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human NBN Polyclonal Antibody (HF552014) is a rabbit polyclonal antibody detecting NBN in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human NBN (Met1-Asp121). | ||||||||
| ターゲット | Nibrin, P95, NBS1, Cell cycle regulatory protein p95, NBS, Nijmegen breakage syndrome protein 1, NBN | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | O60934 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Nibrin (NBN) is a ~84 kDa protein. Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. The MRN complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11, to initiate end resection, which is required for single-strand invasion and recombination. Within the MRN complex, NBN acts as a protein-protein adapter, which specifically recognizes and binds phosphorylated proteins, promoting their recruitment to DNA damage sites. 1. Zhu, XD. et al. (2000) Nature genetics 25, 347-52. PMID: 10888888 2. Stiff, T. et al. (2005) The EMBO journal 24, 199-208. PMID: 15616588 3. Melander, F. et al. (2008) The Journal of cell biology 181, 213-26. PMID: 18411307 4. Chapman, JR. et al. (2008) EMBO reports 9, 795-801. PMID: 18583988 5. Wu, L. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 11200-5. PMID: 18678890 6. Yuan, J. et al. (2009) The Journal of biological chemistry 284, 31746-52. PMID: 19759395 7. Lu, CS. et al. (2012) The Journal of biological chemistry 287, 43984-94. PMID: 23115235 8. Rai, R. et al. (2017) Molecular cell 65, 801-817.e4. PMID: 28216226 9. Myler, LR. et al. (2017) Molecular cell 67, 891-898.e4. PMID: 28867292 10. Trujillo, KM. et al. (1998) The Journal of biological chemistry 273, 21447-50. PMID: 9705271 | ||||||||
| 注意事項 | For research use only. |
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