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Anti-Human NDOR1 Polyclonal Antibody (HP735014)

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Aperçu
Numéro de catalogueHP735014
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human NDOR1 (Met1-Leu154).
Cible NR1, NDOR1, NADPH-dependent diflavin oxidoreductase 1, NADPH-dependent FMN and FAD-containing oxidoreductase, Novel reductase 1
Purification Purified by antigen affinity column.
Numéro d'accès Q9UHB4
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

NADPH-dependent diflavin oxidoreductase 1 (NDOR1) is a ~66 kDa protein. NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of CIAPIN1, another key component of the CIA machinery. In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins. It can also reduce the [2Fe-2S] cluster of CISD1 and activate this protein implicated in Fe/S cluster repair. In vitro can fully activate methionine synthase/MTR in the presence of soluble cytochrome b5/CYB5A.

1. Paine, MJ. et al. (2000) The Journal of biological chemistry 275, 1471-8. PMID: 10625700
2. Finn, RD. et al. (2005) Pharmacogenetics and genomics 15, 381-6. PMID: 15900210
3. Netz, DJ. et al. (2010) Nature chemical biology 6, 758-65. PMID: 20802492
4. Banci, L. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 7136-41. PMID: 23596212
5. Camponeschi, F. et al. (2017) Journal of the American Chemical Society 139, 9479-9482. PMID: 28648056
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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