Anti-Human Nectin-4 Recombinant Antibody (SAA0403) (HV985207)

Name: Anti-Human Nectin-4 Recombinant Antibody (SAA0403)
Brand: abinScience
SKU: HV985207
Price: 191 USD
Availability: InStock

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Даташит
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Обзор

Каталожный номер	HV985207
Реактивность видов	Human
Применения	ELISA, FCM, WB
Хозяинский вид	Mouse
Клоональность	Monoclonal
Изотип	IgG2a, kappa
ID клона	SAA0403
Проверенные приложения	WB, FCM
Цель	LNIR, NECTIN4, PRR4 receptor-related protein 4, Nectin cell adhesion molecule 4, Ig superfamily receptor LNIR, Nectin-4, PVRL4
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>95% as determined by SDS-PAGE.
Очистка	Protein A/G purified from cell culture supernatant.
Номер доступа	Q96NY8
Форма	Liquid
Буфер хранения	0.01M PBS, pH 7.4, 0.09% Sodium azide. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Фон	Nectin-4 (NECTIN4/PVRL4) is a ~55 kDa protein. Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells. NECTIN4 is the therapeutic target of enfortumab vedotin (Padcev).
Примечание	For research use only.

Изображения

Bioactivity

Detects NECTIN4/PVRL4 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human NECTIN4 antibody.NECTIN4 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NECTIN4 antibody monoclonal antibody (Catalog # HV985207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human NECTIN4 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human NECTIN4 monoclonal antibody (Catalog: HV985207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-NECTIN4 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human NECTIN4 (Catalog No: HV985011)
Lane 2: negative control