Anti-Human NEDD4L/Nedd4-2 Polyclonal Antibody (HP349014)

Name: Anti-Human NEDD4L/Nedd4-2 Polyclonal Antibody
Brand: abinScience
SKU: HP349014
Price: 96 USD
Availability: InStock

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Vista general

Número de catálogo

HP349014

Descripción

Anti-Human NEDD4L/Nedd4-2 Polyclonal Antibody (HP349014) is a rabbit polyclonal antibody detecting NEDD4.2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Human, Mouse

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human NEDD4L/Nedd4-2 (Glu629-Asp975).

Objetivo

NEDD4L, E3 ubiquitin-protein ligase NEDD4-like, NEDD4.2, NEDL3, Nedd4-2, HECT-type E3 ubiquitin transferase NED4L, KIAA0439

Purificación

Purified by antigen affinity column.

Número de acceso

Q96PU5

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

E3 ubiquitin-protein ligase NEDD4-like (NEDD4L/Nedd4-2) is a ~111 kDa protein. E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins and is thereby implicated in the regulation of various signaling pathways including autophagy, innate immunity or DNA repair. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Downregulates autophagy and cell growth by ubiquitinating and reducing cellular ULK1 or ASCT2 levels. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, SCN2A/Nav1.2, SCN3A/Nav1.3, SCN5A/Nav1.5, SCN9A/Nav1.7, SCN10A/Nav1.8, KCNA3/Kv1.3, KCNH2, EAAT1, KCNQ2/Kv7.2, KCNQ3/Kv7.3 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2.

1. Kamadurai, HB. et al. (2009) Molecular cell 36, 1095-102. PMID: 20064473

2. Lee, DE. et al. (2020) Cell death & disease 11, 38. PMID: 31959741

3. Gao, P. et al. (2021) Nature communications 12, 1194. PMID: 33608556

4. Kuratomi, G. et al. (2005) The Biochemical journal 386, 461-70. PMID: 15496141

5. Nazio, F. et al. (2017) Cell cycle (Georgetown, Tex.) 16, 1744-1747. PMID: 28820317

6. Kang, Y. et al. (2015) The Biochemical journal 472, 71-82. PMID: 26363003

7. Anta, B. et al. (2016) The Journal of biological chemistry 291, 19132-45. PMID: 27445338

8. Low, LH. et al. (2015) The Journal of biological chemistry 290, 7141-50. PMID: 25631046

9. Yoo, JC. et al. (2013) Journal of dermatological science 72, 246-51. PMID: 23999003

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-Human NEDD4L/Nedd4-2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

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