Anti-Human NEDD4L/Nedd4-2 Polyclonal Antibody (HP349014)

Name: Anti-Human NEDD4L/Nedd4-2 Polyclonal Antibody
Brand: abinScience
SKU: HP349014
Price: 96 USD
Availability: InStock

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Aperçu

Numéro de catalogue

HP349014

Description

Anti-Human NEDD4L/Nedd4-2 Polyclonal Antibody (HP349014) is a rabbit polyclonal antibody detecting NEDD4.2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human NEDD4L/Nedd4-2 (Glu629-Asp975).

Cible

NEDD4L, E3 ubiquitin-protein ligase NEDD4-like, NEDD4.2, NEDL3, Nedd4-2, HECT-type E3 ubiquitin transferase NED4L, KIAA0439

Purification

Purified by antigen affinity column.

Numéro d'accès

Q96PU5

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

E3 ubiquitin-protein ligase NEDD4-like (NEDD4L/Nedd4-2) is a ~111 kDa protein. E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins and is thereby implicated in the regulation of various signaling pathways including autophagy, innate immunity or DNA repair. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Downregulates autophagy and cell growth by ubiquitinating and reducing cellular ULK1 or ASCT2 levels. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, SCN2A/Nav1.2, SCN3A/Nav1.3, SCN5A/Nav1.5, SCN9A/Nav1.7, SCN10A/Nav1.8, KCNA3/Kv1.3, KCNH2, EAAT1, KCNQ2/Kv7.2, KCNQ3/Kv7.3 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2.

1. Kamadurai, HB. et al. (2009) Molecular cell 36, 1095-102. PMID: 20064473

2. Lee, DE. et al. (2020) Cell death & disease 11, 38. PMID: 31959741

3. Gao, P. et al. (2021) Nature communications 12, 1194. PMID: 33608556

4. Kuratomi, G. et al. (2005) The Biochemical journal 386, 461-70. PMID: 15496141

5. Nazio, F. et al. (2017) Cell cycle (Georgetown, Tex.) 16, 1744-1747. PMID: 28820317

6. Kang, Y. et al. (2015) The Biochemical journal 472, 71-82. PMID: 26363003

7. Anta, B. et al. (2016) The Journal of biological chemistry 291, 19132-45. PMID: 27445338

8. Low, LH. et al. (2015) The Journal of biological chemistry 290, 7141-50. PMID: 25631046

9. Yoo, JC. et al. (2013) Journal of dermatological science 72, 246-51. PMID: 23999003

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human NEDD4L/Nedd4-2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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