Anti-Human NEDD4L/Nedd4-2 Polyclonal Antibody (HP349014)

Name: Anti-Human NEDD4L/Nedd4-2 Polyclonal Antibody
Brand: abinScience
SKU: HP349014
Price: 96 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HP349014

説明

Anti-Human NEDD4L/Nedd4-2 Polyclonal Antibody (HP349014) is a rabbit polyclonal antibody detecting NEDD4.2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

種反応性

Human, Mouse

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human NEDD4L/Nedd4-2 (Glu629-Asp975).

ターゲット

NEDD4L, E3 ubiquitin-protein ligase NEDD4-like, NEDD4.2, NEDL3, Nedd4-2, HECT-type E3 ubiquitin transferase NED4L, KIAA0439

精製

Purified by antigen affinity column.

アクセッション番号

Q96PU5

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

E3 ubiquitin-protein ligase NEDD4-like (NEDD4L/Nedd4-2) is a ~111 kDa protein. E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins and is thereby implicated in the regulation of various signaling pathways including autophagy, innate immunity or DNA repair. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Downregulates autophagy and cell growth by ubiquitinating and reducing cellular ULK1 or ASCT2 levels. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, SCN2A/Nav1.2, SCN3A/Nav1.3, SCN5A/Nav1.5, SCN9A/Nav1.7, SCN10A/Nav1.8, KCNA3/Kv1.3, KCNH2, EAAT1, KCNQ2/Kv7.2, KCNQ3/Kv7.3 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2.

1. Kamadurai, HB. et al. (2009) Molecular cell 36, 1095-102. PMID: 20064473

2. Lee, DE. et al. (2020) Cell death & disease 11, 38. PMID: 31959741

3. Gao, P. et al. (2021) Nature communications 12, 1194. PMID: 33608556

4. Kuratomi, G. et al. (2005) The Biochemical journal 386, 461-70. PMID: 15496141

5. Nazio, F. et al. (2017) Cell cycle (Georgetown, Tex.) 16, 1744-1747. PMID: 28820317

6. Kang, Y. et al. (2015) The Biochemical journal 472, 71-82. PMID: 26363003

7. Anta, B. et al. (2016) The Journal of biological chemistry 291, 19132-45. PMID: 27445338

8. Low, LH. et al. (2015) The Journal of biological chemistry 290, 7141-50. PMID: 25631046

9. Yoo, JC. et al. (2013) Journal of dermatological science 72, 246-51. PMID: 23999003

注意事項

For research use only.

イメージ

参考文献

データシート

Anti-Human NEDD4L/Nedd4-2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-Human NEDD4L/Nedd4-2 Polyclonal Antibody (HP349014)

概要

イメージ

参考文献

データシート

SDS

クイックアクセス

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