Anti-Human NEDD4L/Nedd4-2 Polyclonal Antibody (HP349014)

Name: Anti-Human NEDD4L/Nedd4-2 Polyclonal Antibody
Brand: abinScience
SKU: HP349014
Price: 96 USD
Availability: InStock

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Обзор

Каталожный номер

HP349014

Описание

Anti-Human NEDD4L/Nedd4-2 Polyclonal Antibody (HP349014) is a rabbit polyclonal antibody detecting NEDD4.2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human NEDD4L/Nedd4-2 (Glu629-Asp975).

Цель

NEDD4L, E3 ubiquitin-protein ligase NEDD4-like, NEDD4.2, NEDL3, Nedd4-2, HECT-type E3 ubiquitin transferase NED4L, KIAA0439

Очистка

Purified by antigen affinity column.

Номер доступа

Q96PU5

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

E3 ubiquitin-protein ligase NEDD4-like (NEDD4L/Nedd4-2) is a ~111 kDa protein. E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins and is thereby implicated in the regulation of various signaling pathways including autophagy, innate immunity or DNA repair. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Downregulates autophagy and cell growth by ubiquitinating and reducing cellular ULK1 or ASCT2 levels. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, SCN2A/Nav1.2, SCN3A/Nav1.3, SCN5A/Nav1.5, SCN9A/Nav1.7, SCN10A/Nav1.8, KCNA3/Kv1.3, KCNH2, EAAT1, KCNQ2/Kv7.2, KCNQ3/Kv7.3 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2.

1. Kamadurai, HB. et al. (2009) Molecular cell 36, 1095-102. PMID: 20064473

2. Lee, DE. et al. (2020) Cell death & disease 11, 38. PMID: 31959741

3. Gao, P. et al. (2021) Nature communications 12, 1194. PMID: 33608556

4. Kuratomi, G. et al. (2005) The Biochemical journal 386, 461-70. PMID: 15496141

5. Nazio, F. et al. (2017) Cell cycle (Georgetown, Tex.) 16, 1744-1747. PMID: 28820317

6. Kang, Y. et al. (2015) The Biochemical journal 472, 71-82. PMID: 26363003

7. Anta, B. et al. (2016) The Journal of biological chemistry 291, 19132-45. PMID: 27445338

8. Low, LH. et al. (2015) The Journal of biological chemistry 290, 7141-50. PMID: 25631046

9. Yoo, JC. et al. (2013) Journal of dermatological science 72, 246-51. PMID: 23999003

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-Human NEDD4L/Nedd4-2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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