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| カタログ番号 | HA947014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human NEIL3 Polyclonal Antibody (HA947014) is a rabbit polyclonal antibody detecting NEIL3 in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human NEIL3 (Met1-Lys281). | ||||||||
| ターゲット | DNA glycosylase FPG2, NEIL3, Endonuclease 8-like 3, Endonuclease VIII-like 3, Nei-like protein 3, DNA glycosylase/AP lyase Neil3 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q8TAT5 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Endonuclease 8-like 3 (NEIL3) is a ~67 kDa protein. DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). Mediates interstrand cross-link repair in response to replication stress: acts by mediating DNA glycosylase activity, cleaving one of the two N-glycosyl bonds comprising the interstrand cross-link, which avoids the formation of a double-strand break but generates an abasic site that is bypassed by translesion synthesis polymerases. In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. 1. Morland, I. et al. (2002) Nucleic acids research 30, 4926-36. PMID: 12433996 2. Takao, M. et al. (2009) Genes to cells : devoted to molecular & cellular mechanisms 14, 261-70. PMID: 19170771 3. Liu, M. et al. (2012) Protein expression and purification 84, 130-9. PMID: 22569481 4. Krokeide, SZ. et al. (2013) DNA repair 12, 1159-64. PMID: 23755964 | ||||||||
| 注意事項 | For research use only. |
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