Anti-Human NEIL3 Polyclonal Antibody (HA947014)

Name: Anti-Human NEIL3 Polyclonal Antibody
Brand: abinScience
SKU: HA947014
Price: 168 USD
Availability: InStock

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Обзор

Каталожный номер

HA947014

Описание

Anti-Human NEIL3 Polyclonal Antibody (HA947014) is a rabbit polyclonal antibody detecting NEIL3 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Human

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human NEIL3 (Met1-Lys281).

Цель

DNA glycosylase FPG2, NEIL3, Endonuclease 8-like 3, Endonuclease VIII-like 3, Nei-like protein 3, DNA glycosylase/AP lyase Neil3

Очистка

Purified by antigen affinity column.

Номер доступа

Q8TAT5

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Endonuclease 8-like 3 (NEIL3) is a ~67 kDa protein. DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). Mediates interstrand cross-link repair in response to replication stress: acts by mediating DNA glycosylase activity, cleaving one of the two N-glycosyl bonds comprising the interstrand cross-link, which avoids the formation of a double-strand break but generates an abasic site that is bypassed by translesion synthesis polymerases. In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity.

1. Morland, I. et al. (2002) Nucleic acids research 30, 4926-36. PMID: 12433996

2. Takao, M. et al. (2009) Genes to cells : devoted to molecular & cellular mechanisms 14, 261-70. PMID: 19170771

3. Liu, M. et al. (2012) Protein expression and purification 84, 130-9. PMID: 22569481

4. Krokeide, SZ. et al. (2013) DNA repair 12, 1159-64. PMID: 23755964

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-Human NEIL3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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