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Anti-Human NEK1 Polyclonal Antibody (HP702014)

Anti-Human NEK1 Polyclonal Antibody
Anti-Human NEK1 Polyclonal Antibody
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  • 100ug
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Aperçu
Numéro de catalogueHP702014
Description
Anti-Human NEK1 Polyclonal Antibody (HP702014) is a rabbit polyclonal antibody detecting Serine/threonine-protein kinase Nek1; 2.7.11.1; Never in mitosis A-related kinase 1; NimA-related protein kinase 1; Renal carcinoma antigen NY-REN-55; NEK1; KIAA1901. Suitable for Human and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human NEK1 (Cys1177-Glu1258).
Cible Serine/threonine-protein kinase Nek1; 2.7.11.1; Never in mitosis A-related kinase 1; NimA-related protein kinase 1; Renal carcinoma antigen NY-REN-55; NEK1; KIAA1901
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès Q96PY6
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Contexte

Serine/threonine-protein kinase Nek1 is a ~142 kDa protein. Phosphorylates serines and threonines, but also appears to possess tyrosine kinase activity. Involved in DNA damage checkpoint control and for proper DNA damage repair. In response to injury that includes DNA damage, NEK1 phosphorylates VDAC1 to limit mitochondrial cell death. May be implicated in the control of meiosis. Involved in cilium assembly.

1. Chen, Y. et al. (2010) Biochemical and biophysical research communications 394, 798-803. PMID: 20230784
2. Thiel, C. et al. (2011) American journal of human genetics 88, 106-14. PMID: 21211617
Note For research use only
Images
  • Anti-Human NEK1 Polyclonal Antibody

    Western blot

    Lane 1: Mouse brain lysate
    Lane 2: Mouse testis lysate

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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