Anti-Human NHEJ1 Polyclonal Antibody (HP884014)

Name: Anti-Human NHEJ1 Polyclonal Antibody
Brand: abinScience
SKU: HP884014
Price: 168 USD
Availability: InStock

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Precio(USD):

Especificación:

50ug
100ug

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HP884014

Reactividad de especies

Human

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human NHEJ1 (Met1-Ser127).

Objetivo

Non-homologous end-joining factor 1, XRCC4-like factor, NHEJ1, XLF, Protein cernunnos

Purificación

Purified by antigen affinity column.

Número de acceso

Q9H9Q4

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Non-homologous end-joining factor 1 (NHEJ1) is a ~33 kDa protein. DNA repair protein involved in DNA non-homologous end joining (NHEJ); it is required for double-strand break (DSB) repair and V(D)J recombination and is also involved in telomere maintenance. Plays a key role in NHEJ by promoting the ligation of various mismatched and non-cohesive ends. Together with PAXX, collaborates with DNA polymerase lambda (POLL) to promote joining of non-cohesive DNA ends. May act in concert with XRCC5-XRCC6 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are non-complementary or partially complementary. In some studies, has been shown to associate with XRCC4 to form alternating helical filaments that bridge DNA and act like a bandage, holding together the broken DNA until it is repaired.

1. Buck, D. et al. (2006) Cell 124, 287-99. PMID: 16439204

2. Ahnesorg, P. et al. (2006) Cell 124, 301-13. PMID: 16439205

3. Lu, H. et al. (2007) The Journal of biological chemistry 282, 11155-62. PMID: 17317666

4. Tsai, CJ. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 7851-6. PMID: 17470781

5. Gu, J. et al. (2007) Nucleic acids research 35, 5755-62. PMID: 17717001

6. Andres, SN. et al. (2007) Molecular cell 28, 1093-101. PMID: 18158905

7. Yu, Y. et al. (2008) DNA repair 7, 1680-92. PMID: 18644470

8. Malivert, L. et al. (2010) The Journal of biological chemistry 285, 26475-83. PMID: 20558749

9. Roy, S. et al. (2015) Molecular and cellular biology 35, 3017-28. PMID: 26100018

10. Carrillo, J. et al. (2017) Human molecular genetics 26, 1900-1914. PMID: 28369633

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-Human NHEJ1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo