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Anti-Human NKRF Polyclonal Antibody (HT276014)

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Anti-Human NKRF Polyclonal Antibody
Anti-Human NKRF Polyclonal Antibody
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Vista general
Número de catálogoHT276014
Descripción
Anti-Human NKRF Polyclonal Antibody (HT276014) is a rabbit polyclonal antibody detecting NKRF in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human NKRF (Phe162-Thr415).
Objetivo NF-kappa-B-repressing factor, NFkB-repressing factor, NRF, Protein ITBA4, NKRF, ITBA4, NRF
Purificación Purified by antigen affinity column.
Número de acceso O15226
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

NF-kappa-B-repressing factor (NKRF) is a ~77 kDa protein. Enhances the ATPase activity of DHX15 by acting like a brace that tethers mobile sections of DHX15 together, stabilizing a functional conformation with high RNA affinity of DHX15. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription. May also act as a DNA-binding transcription regulator: interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes.

1. Feng, X. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 14212-7. PMID: 12381793
2. Nourbakhsh, M. et al. (1999) The EMBO journal 18, 6415-25. PMID: 10562553
Nota For research use only.
Imágenes
  • Anti-Human NKRF Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-NKRF polyclonal antibody at 1ug/mL on various samples.
    Lane 1: NIH3T3 cell lysate
    Lane 2: Hela cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: MCF-7 cell lysate
    Lane 6: K562 cell lysate
    Lane 7: A549 cell lysate
    Lane 8: Mouse spleen lysate
    Lane 9: Mouse kidney lysate
    Lane 10: Rat spleen lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human NKRF Polyclonal Antibody

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