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| カタログ番号 | HT276014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human NKRF Polyclonal Antibody (HT276014) is a rabbit polyclonal antibody detecting NKRF in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human NKRF (Phe162-Thr415). | ||||||||
| ターゲット | NF-kappa-B-repressing factor, NFkB-repressing factor, NRF, Protein ITBA4, NKRF, ITBA4, NRF | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | O15226 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | NF-kappa-B-repressing factor (NKRF) is a ~77 kDa protein. Enhances the ATPase activity of DHX15 by acting like a brace that tethers mobile sections of DHX15 together, stabilizing a functional conformation with high RNA affinity of DHX15. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription. May also act as a DNA-binding transcription regulator: interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. 1. Feng, X. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 14212-7. PMID: 12381793 2. Nourbakhsh, M. et al. (1999) The EMBO journal 18, 6415-25. PMID: 10562553 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-NKRF polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: Hela cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: MCF-7 cell lysate
Lane 6: K562 cell lysate
Lane 7: A549 cell lysate
Lane 8: Mouse spleen lysate
Lane 9: Mouse kidney lysate
Lane 10: Rat spleen lysate
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