Anti-Human NME1 Polyclonal Antibody (HB870014)

Name: Anti-Human NME1 Polyclonal Antibody
Brand: abinScience
SKU: HB870014
Price: 96 USD
Availability: InStock

Datasheet Compartir producto Copiar información

Precio(USD): --

Especificación:

20ug
50ug
100ug

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HB870014

Descripción

Anti-Human NME1 Polyclonal Antibody (HB870014) is a rabbit polyclonal antibody detecting NME1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human NME1 (Met1-Glu152).

Objetivo

NDPKA, NME1, NDP kinase A, Nucleoside diphosphate kinase A, Granzyme A-activated DNase, NM23, Metastasis inhibition factor nm23, GAAD, Tumor metastatic process-associated protein, NDK A, NM23-H1

Purificación

Purified by antigen affinity column.

Número de acceso

P15531

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Nucleoside diphosphate kinase A (NME1) is a ~17 kDa protein. Catalyzes the transfer of a gamma-phosphoryl group from a nucleoside triphosphate, mainly ATP, to a nucleoside diphosphate via a ping-pong mechanism involving a phosphohistidine intermediate, therefore contributing to the nucleoside triphosphate homeostasis. Also phosphorylates geranyl pyrophosphate (GPP) and farnesyl pyrophosphate (FPP), linking it to isoprenoid metabolism. Additionally, functions as a non-specific serine/threonine kinase and histidine protein kinase, transferring phosphoryl groups from its active site to target proteins. May function as a Mg(2+)-dependent single-stranded DNA endonuclease as part of the SET complex, cooperating with the 3'-5' exonuclease TREX1 to mediate apoptotic DNA fragmentation in cytotoxic T lymphocytes. Reported to nick one DNA strand, enabling TREX1 to remove nucleotides from the free 3' end, enhancing DNA damage and suppressing DNA end reannealing and repair.

1. Wagner, PD. et al. (2000) The Journal of biological chemistry 275, 35570-6. PMID: 10952986

2. Ma, D. et al. (2004) The Journal of biological chemistry 279, 18073-84. PMID: 14960567

3. Yoon, JH. et al. (2005) Biochemistry 44, 15774-86. PMID: 16313181

4. Gilles, AM. et al. (1991) The Journal of biological chemistry 266, 8784-9. PMID: 1851158

5. Kim, MS. et al. (2013) Acta crystallographica. Section D, Biological crystallography 69, 669-80. PMID: 23519676

6. Yu, BYK. et al. (2021) Redox biology 44, 101978. PMID: 33903070

7. MacDonald, NJ. et al. (1996) The Journal of biological chemistry 271, 25107-16. PMID: 8810265

8. Freije, JM. et al. (1997) The Journal of biological chemistry 272, 5525-32. PMID: 9038158

9. Engel, M. et al. (1995) European journal of biochemistry 234, 200-7. PMID: 8529641

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-NME1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse brain lysate
Lane 2: Mouse lung lysate
Lane 3: Mouse liver lysate
Lane 4: Rat brain lysate
Lane 5: Rat lung lysate
Lane 6: Rat liver lysate

Referencias

Datasheet

Anti-Human NME1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-Human NME1 Polyclonal Antibody (HB870014)

Vista general

Imágenes

Referencias

Datasheet

SDS