中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HB870014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human NME1 Polyclonal Antibody (HB870014) is a rabbit polyclonal antibody detecting NME1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
|
||||||||
| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human NME1 (Met1-Glu152). | ||||||||
| ターゲット | NDPKA, NME1, NDP kinase A, Nucleoside diphosphate kinase A, Granzyme A-activated DNase, NM23, Metastasis inhibition factor nm23, GAAD, Tumor metastatic process-associated protein, NDK A, NM23-H1 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P15531 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
||||||||
| 製品使用情報 |
| ||||||||
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Nucleoside diphosphate kinase A (NME1) is a ~17 kDa protein. Catalyzes the transfer of a gamma-phosphoryl group from a nucleoside triphosphate, mainly ATP, to a nucleoside diphosphate via a ping-pong mechanism involving a phosphohistidine intermediate, therefore contributing to the nucleoside triphosphate homeostasis. Also phosphorylates geranyl pyrophosphate (GPP) and farnesyl pyrophosphate (FPP), linking it to isoprenoid metabolism. Additionally, functions as a non-specific serine/threonine kinase and histidine protein kinase, transferring phosphoryl groups from its active site to target proteins. May function as a Mg(2+)-dependent single-stranded DNA endonuclease as part of the SET complex, cooperating with the 3'-5' exonuclease TREX1 to mediate apoptotic DNA fragmentation in cytotoxic T lymphocytes. Reported to nick one DNA strand, enabling TREX1 to remove nucleotides from the free 3' end, enhancing DNA damage and suppressing DNA end reannealing and repair. 1. Wagner, PD. et al. (2000) The Journal of biological chemistry 275, 35570-6. PMID: 10952986 2. Ma, D. et al. (2004) The Journal of biological chemistry 279, 18073-84. PMID: 14960567 3. Yoon, JH. et al. (2005) Biochemistry 44, 15774-86. PMID: 16313181 4. Gilles, AM. et al. (1991) The Journal of biological chemistry 266, 8784-9. PMID: 1851158 5. Kim, MS. et al. (2013) Acta crystallographica. Section D, Biological crystallography 69, 669-80. PMID: 23519676 6. Yu, BYK. et al. (2021) Redox biology 44, 101978. PMID: 33903070 7. MacDonald, NJ. et al. (1996) The Journal of biological chemistry 271, 25107-16. PMID: 8810265 8. Freije, JM. et al. (1997) The Journal of biological chemistry 272, 5525-32. PMID: 9038158 9. Engel, M. et al. (1995) European journal of biochemistry 234, 200-7. PMID: 8529641 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-NME1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse brain lysate
Lane 2: Mouse lung lysate
Lane 3: Mouse liver lysate
Lane 4: Rat brain lysate
Lane 5: Rat lung lysate
Lane 6: Rat liver lysate
お問い合わせ
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
