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| カタログ番号 | HW388107 |
|---|---|
| 種反応性 | Human |
| アプリケーション | FCM, WB |
| 宿主種 | Mouse |
| クローン性 | Monoclonal |
| アイソタイプ | IgG2a, kappa |
| クローンID | SAA0105 |
| ターゲット | Nitric oxide synthase, inducible, NOS2A, Inducible NO synthase, HEP-NOS, Hepatocyte NOS, Peptidyl-cysteine S-nitrosylase NOS2, iNOS, NOS2, Inducible NOS, NOS type II |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >95% as determined by SDS-PAGE. |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | P35228 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| 背景 | Nitric oxide synthase, inducible (NOS2/iNOS) is a ~131 kDa protein. Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8. 1. Charles, IG. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 11419-23. PMID: 7504305 2. Hokari, A. et al. (1994) Journal of biochemistry 116, 575-81. PMID: 7531687 3. Guo, FH. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 7809-13. PMID: 7544004 4. Geller, DA. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 3491-5. PMID: 7682706 5. Jia, J. et al. (2014) Cell 159, 623-34. PMID: 25417112 6. Vuolteenaho, K. et al. (2009) Mediators of inflammation 2009, 345838. PMID: 19688109 |
| 注意事項 | For research use only. |
SDS-PAGE for Anti-Human NOS2/iNOS Antibody (SAA0105)
Western blot analysis was performed using anti-INOS monoclonal antibody at 1ug/mL on various samples.
Lane 1: RAW264.7 cell lystae
Lane 2: RAW264.7+LPS cell lystae
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