Anti-Human NPRL2 Polyclonal Antibody (HP781014)

Name: Anti-Human NPRL2 Polyclonal Antibody
Brand: abinScience
SKU: HP781014
Price: 168 USD
Availability: InStock

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Prix(USD) :

Spécification :

50ug
100ug

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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HP781014

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human NPRL2 (Arg6-Glu161).

Cible

Gene 21 protein, TUSC4, G21 protein, NPRL2, GATOR complex protein NPRL2, Tumor suppressor candidate 4, Nitrogen permease regulator 2-like protein, NPR2-like protein

Purification

Purified by antigen affinity column.

Numéro d'accès

Q8WTW4

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

GATOR1 complex protein NPRL2 is a ~43 kDa protein. Catalytic component of the GATOR1 complex, a multiprotein complex that functions as an inhibitor of the amino acid-sensing branch of the mTORC1 pathway. In response to amino acid depletion, the GATOR1 complex has GTPase activating protein (GAP) activity and strongly increases GTP hydrolysis by RagA/RRAGA (or RagB/RRAGB) within heterodimeric Rag complexes, thereby turning them into their inactive GDP-bound form, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. In the presence of abundant amino acids, the GATOR1 complex is ubiquitinated and inhibited by GATOR2. Within the GATOR1 complex, NPRL2 constitutes the catalytic subunit that mediates the GTPase activator activity and under methionine-sufficient conditions, the GTPase activator activity is inhibited by PRMT1 through methylation and consequently inducing timely mTORC1 activation.

1. Bar-Peled, L. et al. (2013) Science (New York, N.Y.) 340, 1100-6. PMID: 23723238

2. Shen, K. et al. (2018) Nature 556, 64-69. PMID: 29590090

3. Egri, SB. et al. (2022) Molecular cell 82, 1836-1849.e5. PMID: 35338845

4. Jiang, C. et al. (2023) Cell metabolism 35, 2183-2199.e7. PMID: 38006878

5. Jiang, C. et al. (2023) Molecular cell 83, 74-89.e9. PMID: 36528027

6. Weckhuysen, S. et al. (2016) Epilepsia 57, 994-1003. PMID: 27173016

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human NPRL2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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