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| カタログ番号 | HP781014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human NPRL2 (Arg6-Glu161). | ||||||||
| ターゲット | Gene 21 protein, TUSC4, G21 protein, NPRL2, GATOR complex protein NPRL2, Tumor suppressor candidate 4, Nitrogen permease regulator 2-like protein, NPR2-like protein | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q8WTW4 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | GATOR1 complex protein NPRL2 is a ~43 kDa protein. Catalytic component of the GATOR1 complex, a multiprotein complex that functions as an inhibitor of the amino acid-sensing branch of the mTORC1 pathway. In response to amino acid depletion, the GATOR1 complex has GTPase activating protein (GAP) activity and strongly increases GTP hydrolysis by RagA/RRAGA (or RagB/RRAGB) within heterodimeric Rag complexes, thereby turning them into their inactive GDP-bound form, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. In the presence of abundant amino acids, the GATOR1 complex is ubiquitinated and inhibited by GATOR2. Within the GATOR1 complex, NPRL2 constitutes the catalytic subunit that mediates the GTPase activator activity and under methionine-sufficient conditions, the GTPase activator activity is inhibited by PRMT1 through methylation and consequently inducing timely mTORC1 activation. 1. Bar-Peled, L. et al. (2013) Science (New York, N.Y.) 340, 1100-6. PMID: 23723238 2. Shen, K. et al. (2018) Nature 556, 64-69. PMID: 29590090 3. Egri, SB. et al. (2022) Molecular cell 82, 1836-1849.e5. PMID: 35338845 4. Jiang, C. et al. (2023) Cell metabolism 35, 2183-2199.e7. PMID: 38006878 5. Jiang, C. et al. (2023) Molecular cell 83, 74-89.e9. PMID: 36528027 6. Weckhuysen, S. et al. (2016) Epilepsia 57, 994-1003. PMID: 27173016 | ||||||||
| 注意事項 | For research use only. |
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