Anti-Human NPRL2 Polyclonal Antibody (HP781014)

Name: Anti-Human NPRL2 Polyclonal Antibody
Brand: abinScience
SKU: HP781014
Price: 168 USD
Availability: InStock

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50ug
100ug

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HP781014

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human NPRL2 (Arg6-Glu161).

Цель

Gene 21 protein, TUSC4, G21 protein, NPRL2, GATOR complex protein NPRL2, Tumor suppressor candidate 4, Nitrogen permease regulator 2-like protein, NPR2-like protein

Очистка

Purified by antigen affinity column.

Номер доступа

Q8WTW4

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

GATOR1 complex protein NPRL2 is a ~43 kDa protein. Catalytic component of the GATOR1 complex, a multiprotein complex that functions as an inhibitor of the amino acid-sensing branch of the mTORC1 pathway. In response to amino acid depletion, the GATOR1 complex has GTPase activating protein (GAP) activity and strongly increases GTP hydrolysis by RagA/RRAGA (or RagB/RRAGB) within heterodimeric Rag complexes, thereby turning them into their inactive GDP-bound form, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. In the presence of abundant amino acids, the GATOR1 complex is ubiquitinated and inhibited by GATOR2. Within the GATOR1 complex, NPRL2 constitutes the catalytic subunit that mediates the GTPase activator activity and under methionine-sufficient conditions, the GTPase activator activity is inhibited by PRMT1 through methylation and consequently inducing timely mTORC1 activation.

1. Bar-Peled, L. et al. (2013) Science (New York, N.Y.) 340, 1100-6. PMID: 23723238

2. Shen, K. et al. (2018) Nature 556, 64-69. PMID: 29590090

3. Egri, SB. et al. (2022) Molecular cell 82, 1836-1849.e5. PMID: 35338845

4. Jiang, C. et al. (2023) Cell metabolism 35, 2183-2199.e7. PMID: 38006878

5. Jiang, C. et al. (2023) Molecular cell 83, 74-89.e9. PMID: 36528027

6. Weckhuysen, S. et al. (2016) Epilepsia 57, 994-1003. PMID: 27173016

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-Human NPRL2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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