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Anti-Human NR1D1 Polyclonal Antibody (HB802014)

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Anti-Human NR1D1 Polyclonal Antibody
Anti-Human NR1D1 Polyclonal Antibody
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Vista general
Número de catálogoHB802014
Descripción
Anti-Human NR1D1 Polyclonal Antibody (HB802014) is a rabbit polyclonal antibody detecting NR1D1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human NR1D1 (Thr431-Gln614).
Objetivo THRAL, EAR1, EAR-1, NR1D1, Rev-erbA-alpha, Nuclear receptor subfamily 1 group D member 1, HREV, V-erbA-related protein 1
Purificación Purified by antigen affinity column.
Número de acceso P20393
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Nuclear receptor subfamily 1 group D member 1 (NR1D1) is a ~66 kDa protein. Transcriptional repressor which coordinates circadian rhythm and metabolic pathways in a heme-dependent manner. Integral component of the complex transcription machinery that governs circadian rhythmicity and forms a critical negative limb of the circadian clock by directly repressing the expression of core clock components BMAL1, CLOCK and CRY1. Also regulates genes involved in metabolic functions, including lipid and bile acid metabolism, adipogenesis, gluconeogenesis and the macrophage inflammatory response. Acts as a receptor for heme which stimulates its interaction with the NCOR1/HDAC3 corepressor complex, enhancing transcriptional repression. Recognizes two classes of DNA response elements within the promoter of its target genes and can bind to DNA as either monomers or homodimers, depending on the nature of the response element.

Nota For research use only.
Imágenes
  • Anti-Human NR1D1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-NR1D1 polyclonal antibody at 1µg/ml on various samples.
    Lane 1: Mouse skeletal muscle lysate
    Lane 2: Rat lung lysate
    Lane 3: Rat liver lysate
    Lane 4: Rat skeletal muscle lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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