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Anti-Human NSUN5 Polyclonal Antibody (HP203014)

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Aperçu
Numéro de catalogueHP203014
Description
Anti-Human NSUN5 Polyclonal Antibody (HP203014) is a rabbit polyclonal antibody detecting NSUN5 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human NSUN5 (Met1-Arg429).
Cible NOL1R, NOL1-related protein, NSUN5, 28S rRNA (cytosine-C(5))-methyltransferase, WBSCR20A, NOL1/NOP2/Sun domain family member 5, WBSCR20, NSUN5A, Williams-Beuren syndrome chromosomal region 20A protein
Purification Purified by antigen affinity column.
Numéro d'accès Q96P11
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

28S rRNA (cytosine-C(5))-methyltransferase (NSUN5) is a ~46 kDa protein. S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C(5) position of cytosine 3782 (m5C3782) in 28S rRNA. m5C3782 promotes protein translation without affecting ribosome biogenesis and fidelity. Required for corpus callosum and cerebral cortex development.

1. Sharma, S. et al. (2013) Nucleic acids research 41, 9062-76. PMID: 23913415
2. Janin, M. et al. (2019) Acta neuropathologica 138, 1053-1074. PMID: 31428936
3. Heissenberger, C. et al. (2019) Nucleic acids research 47, 11807-11825. PMID: 31722427
Note For research use only.
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Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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