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Anti-Human NUDT3 Polyclonal Antibody (HF767014)

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Número de catálogoHF767014
Descripción
Anti-Human NUDT3 Polyclonal Antibody (HF767014) is a rabbit polyclonal antibody detecting NUDT3 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human NUDT3 (Gly16-Ser163).
Objetivo Diphosphoinositol polyphosphate phosphohydrolase 1, DIPP-1, 3.6.1.52, Diadenosine hexaphosphate hydrolase, Ap6A hydrolase, 3.6.1.61, Endopolyphosphatase, 3.6.1.10, Nucleoside diphosphate-linked moiety X motif 3, Nudix motif 3, m7GpppN-mRNA hydrolase, 3.6.1.62, m7GpppX diphosphatase, 3.6.1.59, NUDT3, DIPP, DIPP1
Purificación Purified by antigen affinity column.
Número de acceso O95989
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Diphosphoinositol polyphosphate phosphohydrolase 1 (NUDT3) is a ~19 kDa protein. Cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate) and [PP]2-InsP4 (bisdiphosphoinositol tetrakisphosphate), suggesting that it may play a role in signal transduction. InsP6 (inositol hexakisphosphate) is not a substrate. Acts as a negative regulator of the ERK1/2 pathway. Also able to catalyze the hydrolysis of dinucleoside oligophosphates, with diadenosine 5',5'''-P1,P6-hexaphosphate (Ap6A) and diadenosine 5',5'''- P1,P5-pentaphosphate (Ap5A) being the preferred substrates. The major reaction products are ADP and p4a from Ap6A and ADP and ATP from Ap5A.

1. Yang, X. et al. (1999) The Journal of biological chemistry 274, 35434-40. PMID: 10585413
2. Fisher, DI. et al. (2002) The Journal of biological chemistry 277, 47313-7. PMID: 12370170
3. Safrany, ST. et al. (1998) The EMBO journal 17, 6599-607. PMID: 9822604
4. Safrany, ST. et al. (1999) The Journal of biological chemistry 274, 21735-40. PMID: 10419486
5. Grudzien-Nogalska, E. et al. (2016) RNA (New York, N.Y.) 22, 773-81. PMID: 26932476
6. Sahu, S. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 19245-19253. PMID: 32727897
Nota For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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