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Anti-Human PDE5A Polyclonal Antibody (HF577014)

Anti-Human PDE5A Polyclonal Antibody
Anti-Human PDE5A Polyclonal Antibody
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Aperçu
Numéro de catalogueHF577014
Description
Anti-Human PDE5A Polyclonal Antibody (HF577014) is a rabbit polyclonal antibody detecting PDE5A in ELISA, IHC, WB. Suitable for Rattus norvegicus, Mus musculus, Human, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human PDE5A (Glu321-Lys482).
Cible PDE5A
Purification Purified by antigen affinity column.
Numéro d'accès O76074
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifscGMP-specific 3', 5'-cyclic phosphodiesterase, 3.1.4.35, cGMP-binding cGMP-specific phosphodiesterase, CGB-PDE, PDE5A, PDE5
Contexte

cGMP-specific 3',5'-cyclic phosphodiesterase (PDE5A) is a ~99 kDa protein. Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Specifically regulates nitric-oxide-generated cGMP.

1. Gerhard, DS. et al. (2004) Genome research 14, 2121-7. PMID: 15489334
2. Loughney, K. et al. (1998) Gene 216, 139-47. PMID: 9714779
Note For research use only.
Images
  • Anti-Human PDE5A Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PED5A polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse lung lysate
    Lane 2: Rat lung lysate

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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