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Anti-Human PDGF-B Recombinant Antibody (SAA0449), FITC (HF877117)

Anti-Human PDGF-B Recombinant Antibody (SAA0449), FITC
Anti-Human PDGF-B Recombinant Antibody (SAA0449), FITC
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  • 100T
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Vista general
Número de catálogoHF877117
Reactividad de especiesHuman
AplicacionesELISA, FCM
Especie huéspedMouse
ClonalidadMonoclonal
IsotipoIgG2a, kappa
ID de clonSAA0449
ConjugaciónFITC
Objetivo PDGF-2, Proto-oncogene c-Sis, PDGF2, SIS, Platelet-derived growth factor B chain, PDGFB, Becaplermin, Platelet-derived growth factor beta polypeptide, Platelet-derived growth factor subunit B, PDGF subunit B
Nivel de endotoxinas < 10 EU/mg
Pureza >90% as determined by SDS-PAGE.
Productos asociados Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173), FITC
Número de acceso P01127
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Estabilidad y almacenamiento Store at 4°C for 12 months. Protect from light. Do not freeze.
Fondo

Platelet-derived growth factor subunit B (PDGFB) is a ~27 kDa protein. Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing.

1. Vanlandewijck, M. et al. (2015) PloS one 10, e0143407. PMID: 26599395
Nota For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Imágenes
  • Anti-Human PDGF-B Recombinant Antibody (SAA0449), FITC

    Flow CytoMetry

    Flow-cytometry using PE anti-human PDGFB antibody. MCF-7 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human PDGFB monoclonal antibody (Catalog: HF877117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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