Anti-Human PELO Polyclonal Antibody (HP640014)

Name: Anti-Human PELO Polyclonal Antibody
Brand: abinScience
SKU: HP640014
Price: 96 USD
Availability: InStock

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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HP640014

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human PELO (Met1-Asp385).

Cible

PELO, Protein pelota homolog

Purification

Purified by antigen affinity column.

Numéro d'accès

Q9BRX2

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Protein pelota homolog is a ~43 kDa protein. Component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway. In the Pelota-HBS1L complex, PELO recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel. Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway. As part of the PINK1-regulated signaling, upon mitochondrial damage is recruited to the ribosome/mRNA-ribonucleoprotein complex associated to mitochondrial outer membrane thereby enabling the recruitment of autophagy receptors and induction of mitophagy.

1. Pisareva, VP. et al. (2011) The EMBO journal 30, 1804-17. PMID: 21448132

2. Saito, S. et al. (2013) The Journal of biological chemistry 288, 17832-43. PMID: 23667253

3. Ikeuchi, K. et al. (2016) FEBS letters 590, 3254-63. PMID: 27543824

4. Shao, S. et al. (2016) Cell 167, 1229-1240.e15. PMID: 27863242

5. Zinoviev, A. et al. (2020) Molecular cell 77, 1340-1349.e6. PMID: 32006463

6. Wu, Z. et al. (2018) Cell metabolism 28, 130-144.e7. PMID: 29861391

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PELO polyclonal antibody at 1μg/ml on various samples.
Lane 1: RAW264.7 cell lysate

Reférences

Fiche technique

Anti-Human PELO Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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