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| カタログ番号 | HP640014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human PELO (Met1-Asp385). | ||||||||
| ターゲット | PELO, Protein pelota homolog | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q9BRX2 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Protein pelota homolog is a ~43 kDa protein. Component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway. In the Pelota-HBS1L complex, PELO recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel. Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway. As part of the PINK1-regulated signaling, upon mitochondrial damage is recruited to the ribosome/mRNA-ribonucleoprotein complex associated to mitochondrial outer membrane thereby enabling the recruitment of autophagy receptors and induction of mitophagy. 1. Pisareva, VP. et al. (2011) The EMBO journal 30, 1804-17. PMID: 21448132 2. Saito, S. et al. (2013) The Journal of biological chemistry 288, 17832-43. PMID: 23667253 3. Ikeuchi, K. et al. (2016) FEBS letters 590, 3254-63. PMID: 27543824 4. Shao, S. et al. (2016) Cell 167, 1229-1240.e15. PMID: 27863242 5. Zinoviev, A. et al. (2020) Molecular cell 77, 1340-1349.e6. PMID: 32006463 6. Wu, Z. et al. (2018) Cell metabolism 28, 130-144.e7. PMID: 29861391 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-PELO polyclonal antibody at 1μg/ml on various samples.
Lane 1: RAW264.7 cell lysate
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