Anti-Human PELO Polyclonal Antibody (HP640014)

Name: Anti-Human PELO Polyclonal Antibody
Brand: abinScience
SKU: HP640014
Price: 96 USD
Availability: InStock

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HP640014

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human PELO (Met1-Asp385).

Цель

PELO, Protein pelota homolog

Очистка

Purified by antigen affinity column.

Номер доступа

Q9BRX2

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Protein pelota homolog is a ~43 kDa protein. Component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway. In the Pelota-HBS1L complex, PELO recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel. Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway. As part of the PINK1-regulated signaling, upon mitochondrial damage is recruited to the ribosome/mRNA-ribonucleoprotein complex associated to mitochondrial outer membrane thereby enabling the recruitment of autophagy receptors and induction of mitophagy.

1. Pisareva, VP. et al. (2011) The EMBO journal 30, 1804-17. PMID: 21448132

2. Saito, S. et al. (2013) The Journal of biological chemistry 288, 17832-43. PMID: 23667253

3. Ikeuchi, K. et al. (2016) FEBS letters 590, 3254-63. PMID: 27543824

4. Shao, S. et al. (2016) Cell 167, 1229-1240.e15. PMID: 27863242

5. Zinoviev, A. et al. (2020) Molecular cell 77, 1340-1349.e6. PMID: 32006463

6. Wu, Z. et al. (2018) Cell metabolism 28, 130-144.e7. PMID: 29861391

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-PELO polyclonal antibody at 1μg/ml on various samples.
Lane 1: RAW264.7 cell lysate

Ссылки

Даташит

Anti-Human PELO Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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