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Anti-Human PGAM1 Polyclonal Antibody (HB661014)

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Anti-Human PGAM1 Polyclonal Antibody
Anti-Human PGAM1 Polyclonal Antibody
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Vista general
Número de catálogoHB661014
Descripción
Anti-Human PGAM1 Polyclonal Antibody (HB661014) is a rabbit polyclonal antibody detecting PGAM1 in ELISA, IHC, WB. Suitable for Rattus norvegicus, Pan troglodytes, Human, Gallus gallus, and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human PGAM1 (Met1-Lys254).
Objetivo PGAM1
Purificación Purified by antigen affinity column.
Número de acceso P18669
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativosPhosphoglycerate mutase 1, 5.4.2.11, 5.4.2.4, BPG-dependent PGAM 1, Phosphoglycerate mutase isozyme B, PGAM-B, PGAM1, PGAMA, CDABP0006
Fondo

Phosphoglycerate mutase 1 (PGAM1) is a ~28 kDa protein. Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate, a crucial step in glycolysis, by using 2,3-bisphosphoglycerate. Also catalyzes the interconversion of (2R)-2,3-bisphosphoglycerate and (2R)-3-phospho-glyceroyl phosphate.

1. Hitosugi, T. et al. (2013) Nature communications 4, 1790. PMID: 23653202
Nota For research use only.
Imágenes
  • Anti-Human PGAM1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PAGM1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: NIH3T3 cell lysate
    Lane 2: Hela cell lysate
    Lane 3: 293T cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: Lncap cell lysate
    Lane 6: K562 cell lysate
    Lane 7: U2OS cell lysate
    Lane 8: 4T1 cell lysate
    Lane 9: HepG2 cell lysate
    Lane 10: Mouse liver lysate
    Lane11: Mouse kidney lysate
    Lane12: Rat liver lysate
    Lane13: Rat kidney lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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