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| カタログ番号 | HA211014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human PHF5A Polyclonal Antibody (HA211014) is a rabbit polyclonal antibody detecting PHF5A in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human PHF5A (Arg21-Arg110). | ||||||||
| ターゲット | PHD finger-like domain-containing protein 5A, PHD finger-like domain protein 5A, Splicing factor 3B-associated 14 kDa protein, SF3b14b, PHF5A | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q7RTV0 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | PHD finger-like domain-containing protein 5A (PHF5A) is a ~12 kDa protein. Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, PHF5A is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs. 1. Will, CL. et al. (2002) The EMBO journal 21, 4978-88. PMID: 12234937 2. Cretu, C. et al. (2016) Molecular cell 64, 307-319. PMID: 27720643 3. Teng, T. et al. (2017) Nature communications 8, 15522. PMID: 28541300 4. Zhang, Z. et al. (2020) Nature 583, 310-313. PMID: 32494006 5. Tholen, J. et al. (2022) Science (New York, N.Y.) 375, 50-57. PMID: 34822310 | ||||||||
| 注意事項 | For research use only. |
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