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Каталожный номерHA211014
Описание
Anti-Human PHF5A Polyclonal Antibody (HA211014) is a rabbit polyclonal antibody detecting PHF5A in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовHuman, Mouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human PHF5A (Arg21-Arg110).
Цель PHD finger-like domain-containing protein 5A, PHD finger-like domain protein 5A, Splicing factor 3B-associated 14 kDa protein, SF3b14b, PHF5A
Очистка Purified by antigen affinity column.
Номер доступа Q7RTV0
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

PHD finger-like domain-containing protein 5A (PHF5A) is a ~12 kDa protein. Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, PHF5A is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs.

1. Will, CL. et al. (2002) The EMBO journal 21, 4978-88. PMID: 12234937
2. Cretu, C. et al. (2016) Molecular cell 64, 307-319. PMID: 27720643
3. Teng, T. et al. (2017) Nature communications 8, 15522. PMID: 28541300
4. Zhang, Z. et al. (2020) Nature 583, 310-313. PMID: 32494006
5. Tholen, J. et al. (2022) Science (New York, N.Y.) 375, 50-57. PMID: 34822310
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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