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Anti-Human PNPLA3 Polyclonal Antibody (HA111014)

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Aperçu
Numéro de catalogueHA111014
Description
Anti-Human PNPLA3 Polyclonal Antibody (HA111014) is a rabbit polyclonal antibody detecting PNPLA3 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human PNPLA3 (Met1-Lys252).
Cible 1-acylglycerol-3-phosphate O-acyltransferase PNPLA3, iPLA2-epsilon, C22orf20, Lysophosphatidic acid acyltransferase, Acylglycerol transacylase, Calcium-independent phospholipase A2-epsilon, Patatin-like phospholipase domain-containing protein 3, ADPN, Adiponutrin, PNPLA3
Purification Purified by antigen affinity column.
Numéro d'accès Q9NST1
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

1-acylglycerol-3-phosphate O-acyltransferase PNPLA3 is a ~52 kDa protein. Specifically catalyzes coenzyme A (CoA)-dependent acylation of 1-acyl-sn-glycerol 3-phosphate (2-lysophosphatidic acid/LPA) to generate phosphatidic acid (PA), an important metabolic intermediate and precursor for both triglycerides and glycerophospholipids. Does not esterify other lysophospholipids. Acyl donors are long chain (at least C16) fatty acyl-CoAs: arachidonoyl-CoA, linoleoyl-CoA, oleoyl-CoA and at a lesser extent palmitoyl-CoA. Additionally possesses low triacylglycerol lipase and CoA-independent acylglycerol transacylase activities and thus may play a role in acyl-chain remodeling of triglycerides. In vitro may express hydrolytic activity against glycerolipids triacylglycerol, diacylglycerol and monoacylglycerol, with a strong preference for oleic acid as the acyl moiety.

1. Kumari, M. et al. (2012) Cell metabolism 15, 691-702. PMID: 22560221
2. Jenkins, CM. et al. (2004) The Journal of biological chemistry 279, 48968-75. PMID: 15364929
3. He, S. et al. (2010) The Journal of biological chemistry 285, 6706-15. PMID: 20034933
4. Huang, Y. et al. (2011) The Journal of biological chemistry 286, 37085-93. PMID: 21878620
Note For research use only.
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Reférences
Fiche technique
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human PNPLA3 Polyclonal Antibody

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