Anti-Human POLB Polyclonal Antibody (HY116014)

Name: Anti-Human POLB Polyclonal Antibody
Brand: abinScience
SKU: HY116014
Price: 168 USD
Availability: InStock

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Precio(USD):

Especificación:

50ug
100ug

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HY116014

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human POLB (Asn12-Glu335).

Objetivo

DNA polymerase beta, POLB

Purificación

Purified by antigen affinity column.

Número de acceso

P06746

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

DNA polymerase beta (POLB) is a ~38 kDa protein. Repair polymerase that plays a key role in base-excision repair. During this process, the damaged base is excised by specific DNA glycosylases, the DNA backbone is nicked at the abasic site by an apurinic/apyrimidic (AP) endonuclease, and POLB removes 5'-deoxyribose-phosphate from the preincised AP site acting as a 5'-deoxyribose-phosphate lyase (5'-dRP lyase); through its DNA polymerase activity, it adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. It is also able to cleave sugar-phosphate bonds 3' to an intact AP site, acting as an AP lyase.

1. Iwanaga, A. et al. (1999) Mutation research 435, 121-8. PMID: 10556592

2. Bennett, RA. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 7166-9. PMID: 9207062

3. Matsumoto, Y. et al. (1998) Biochemistry 37, 6456-64. PMID: 9572863

4. Lang, T. et al. (2007) Molecular and cellular biology 27, 5587-96. PMID: 17526740

5. Prasad, R. et al. (1998) The Journal of biological chemistry 273, 11121-6. PMID: 9556598

6. Prasad, R. et al. (1998) The Journal of biological chemistry 273, 15263-70. PMID: 9614142

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-Human POLB Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo