Anti-Human POLQ Polyclonal Antibody (HF853014)

Name: Anti-Human POLQ Polyclonal Antibody
Brand: abinScience
SKU: HF853014
Price: 168 USD
Availability: InStock

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Especificación:

50ug
100ug

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Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HF853014

Descripción

Anti-Human POLQ Polyclonal Antibody (HF853014) is a rabbit polyclonal antibody detecting POLQ in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Human

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human POLQ (Met1-Met894).

Objetivo

POLH, DNA polymerase eta, DNA polymerase theta, POLQ

Purificación

Purified by antigen affinity column.

Número de acceso

O75417

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

DNA polymerase theta (POLQ) is a ~289 kDa protein. Low-fidelity DNA polymerase with a helicase activity that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery required to repair double-strand breaks in DNA during mitosis. MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation. MMEJ is required during mitosis to repair persistent double-strand breaks that originate in S-phase. Although error-prone, MMEJ protects against chromosomal instability and tumorigenesis. The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs.

1. Seki, M. et al. (2003) Nucleic acids research 31, 6117-26. PMID: 14576298

2. Arana, ME. et al. (2008) Nucleic acids research 36, 3847-56. PMID: 18503084

3. Yoon, JH. et al. (2014) The Journal of biological chemistry 289, 13177-85. PMID: 24648516

4. Ceccaldi, R. et al. (2015) Nature 518, 258-62. PMID: 25642963

5. Kent, T. et al. (2015) Nature structural & molecular biology 22, 230-7. PMID: 25643323

6. Zahn, KE. et al. (2015) Nature structural & molecular biology 22, 304-11. PMID: 25775267

7. Newman, JA. et al. (2015) Structure (London, England : 1993) 23, 2319-2330. PMID: 26636256

8. Kent, T. et al. (2016) eLife 5. PMID: 27311885

9. Kent, T. et al. (2016) Nucleic acids research 44, 9381-9392. PMID: 27591252

10. Wang, Z. et al. (2019) The Journal of biological chemistry 294, 3909-3919. PMID: 30655289

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-Human POLQ Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

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